Selective Determination of Nanogram γ-Globulin in Human Blood Serum by Resonance Light Scattering of Functionalized Nano-PbS

Functionalized nano-PbS has been prepared and characterized. The functionalized nanoparticles have good dispersibility in water. Reaction of functionalized nano-PbS with γ-globulin (γ-IgG) results an enhanced resonance light scattering (RLS) around 385nm.However, when the content of HSA is lower than 0.5μg/ml^-1 the RLS enhancement is very weak and is nonlinear to concentration of HSA. Based on these results, a new direct quantitative determination method for γ-globulin in blood serum samples without separation is established.Under optimal conditions, the enhanced RLS intensity is in proportion to the γ-IgG concentration in the range 10-500ng/mL. The limit of detection is 2.75ng/mL. This method is proved to be very sensitive, rapid, simple and selective for detection of γ-IgG in blood serum.     

Influence of pyridine oximate and quaternized pyridinium oximate ions on the hydrolysis of phosphate esters in cationic microemulsions

Kinetic studies have been performed to understand the hydrolytic potencies of oximate (2- and 4-pyridinealdoxime) and its functionalized oximate (4-(hydroxyiminomethyl)-1-alkylpyridinium bromide) ions (alkyl?=?C₁₀H₂₁ (4-C₁₀PyOx^-); alkyl?=?C₁₂H₂₅ (4-C₁₂PyOx^-)) in the cleavage of phosphate esters, diethyl p-nitrophenylphosphate (Paraoxon) and p-nitrophenyl diphenyl phosphate (PNPDPP) in a cationic (O/W) microemulsion system (ME) over a pH range 7.5 to 11.0 at 300?K. The k_obs values for the reaction of paraoxon with oximate and its functionalized oximate were determined in different microemulsion composition and the kinetic rate data shows that k_obs values increases with increasing water content. The specificity of different chain length of alcohols (n-butanol, n-pentanol, n-hexanol and n-octanol) was also investigated in hydrolytic reactions of paraoxon for different microemulsion composition.     

官能团化N,C双齿N-杂环卡宾配合物研究进展

综述了官能团化N,C双齿N-杂环卡宾金属配合物研究的最新进展,着重讨论了这些配合物对各种化学反应的催化性能.     

硒功能化杯芳烃载体银离子选择性膜电极的研究

合成6个硒功能化杯芳烃为载体,采用双层夹心膜电位法直接测定了溶剂聚合物膜中载体与金属离子生成的络合物生成常数。制备并考察了以硒功能化杯芳烃为载体的银离子选择性电极的性能,发现以载体6为银离子选择性电子的载体,制成的膜电极,用作Ag^ 电位滴定Cl^-的指示电极,效果最佳。     

Complexing Polymer Films in The Preparation of Modified Electrodes for Detection of Metal Ions

Summary: The complexing properties of poly (3-(pyrrol-1-yl)propylmalonic acid) (poly1) and poly(N,N′-ethylenebis[N-[(3-(pyrrole-1-yl)propyl) carbamoyl) methyl]-glycine (poly2) coated electrodes (C|poly1 and C|poly2) towards Cu(II), Pb(II), Hg(II) and Cd(II) cations using the open circuit chemical preconcentration-anodic stripping technique were studied. Sorption process of metal cations onto complexing surfaces was readily investigated through the combination of a chemical pre-concentration-anodic stripping technique with a Langmuir isotherm model. The modified electrodes were used for the voltammetric determination of Cu(II), Pb(II), Hg(II) and Cd(II) ions, giving low detection limits for Cu(II) (5 × 10⁻⁹ mol L⁻¹) and Pb(II) (5 × 10⁻¹⁰ mol L⁻¹). The ability of the modified electrodes to analyze Cu(II) ions in natural sample has been demonstrated by the analysis of a tap water sample. The results of the preconcentration process under competitive conditions clearly shows that the selectivity of complexing molecular electrode materials can be subtly tuned upon playing on the accumulation time, polymer thickness and/or memory effect of the binding polymers, opening up new avenues towards evolutive and efficient smart sensing materials.     

酸性功能化离子液体催化“一锅法”合成酰胺烷基萘酚

用磺酸型离子液体1-甲基-3-磺酸丙基咪唑硫酸氢盐([MIMPS][HSO₄])催化β-萘酚、芳香醛和酰胺（或脲）3组分“一锅法” 类Ritter反应合成了酰胺烷基萘酚。 投料比为n(β-萘酚)∶n(芳香醛)∶n(酰胺或脲)∶n([MIMPS][HSO₄])=1∶1∶1.3∶0.1,无需溶剂,恒温125 ℃反应5～40 min,通过简单水洗抽滤即可分离产物,酰胺烷基萘酚产率为85%～97%。 离子液体[MIMPS][HSO₄]可回收重复使用4次,其催化活性无明显降低。 探讨了醛、酰胺或脲结构对反应的影响及可能的反应机理。     

Preparation of chitosan functionalized monolithic silica column for hydrophilic interaction liquid chromatography

A novel cationic hydrophilic interaction monolithic stationary phase based on the chemical modification of carboxymethyl chitosan (CMCH) to the monolithic silica skeleton using carbodiimide as an activation reagent was prepared for performing capillary liquid chromatography. The amino and hydroxy moieties of CMCH functioned as both the ion-exchange sites and polar providers. The performance of the column was studied by the separation of polar acidic compounds. The chitosan functionalized monolithic silica column showed good selectivity for nucleosides, nucleotides, aromatic acids and aliphatic acids. The mechanism for the separation of these compounds was also studied. The results showed that these compounds were separated primarily based on the hydrophilic interaction mechanism.     

Amine-terminated organosilica nanosphere functionalized prussian blue for the electrochemical detection of glucose

A new glucose biosensor had been developed by immobilizing positively charged gold nanoparticles (PGNs) on organosilica nanosphere functionalized prussian blue (OSiFPB)-modified gold electrode. The OSiFPB compound could not only effectively prevent the leakage of the PB mediator during measurements, but also easily form stable film on the electrode surface with efficient redox-activity and excellent conductivity. Furthermore, with the negatively charged surface of OSiFPB, this film could be used as an interface to adsorb the PGNs, which provided a congenial microenvironment for adsorbing biomolecules and decreased the electron-transfer impedance. So, with glucose oxidase as a model biomolecular, the proposed sensor showed rapid and highly sensitive amperometric response to glucose and this immobilization approach effectively improved the stability of the electron-transfer mediator. This work would be promising for construction of biosensor and bioelectronic devices.     

工科基础物理实验课程教学改革与实践

提出了模块化教学体系的新思路及具体实施方案。在教学上将传统内容与现代高新技术相互结合;教学方法上将程序式与多元式相结合,注重了基本技能、实验总结能力和创新精神的培养。总结了试点以来取得的教学经验和体会,并对不足之处进行了分析。     

Chain Walking Ethylene Copolymerization with an ATRP Inimer for One‐Pot Synthesis of Hyperbranched Polyethylenes Tethered with ATRP Initiating Sites

A one‐pot procedure for the synthesis of hyperbranched polyethylenes tethered with ATRP initiating sites by chain walking ethylene copolymerization with an acrylate‐type ATRP inimer, 2‐(2‐bromoisobutyryloxy) ethyl acrylate (BIEA) is reported. Because of its ability to incorporate acrylate‐type comonomers and tolerance toward the α‐bromoester group, the chain walking Pd‐diimine catalyst, [(ArNC(Me) (Me)CNAr)Pd(CH₃)(NCMe)]SbF₆ (Ar = 2,6‐(iPr)₂C₆H₃), allowed the successful synthesis of a series of hyperbranched copolymers tethered with 2‐bromoisobutyryl groups at different densities. These copolymers may serve as polyfunctional macroinitiators for the ATRP of functional monomers to further synthesize core‐shell structured functionalized copolymers with a hyperbranched polyethylene core grafted with side chains of the functional monomers.